Description

This 3D model depicts a diagnostic Immunoglobulin G (IgG) antibody conjugated to the Omega subunit of beta-galactosidase (the Enzyme Acceptor). The omega fragment is a large, inactive polypeptide (approx. 113kDa 997 residues) derived from the LacZ gene. In the ESSENCE platform, this conjugate acts as the primary capture and reporting component. When the target biomarker brings this omega-conjugate into proximity with its complementary alpha-peptide partner, the beta-galactosidase enzyme is reconstituted into its active tetrameric form, facilitating the hydrolysis of colorimetric substrates (e.g., X-gal or CPRG). Structural Note: Rendered as a high-fidelity surface mesh, this model highlights the significant size of the omega domain relative to the IgG, which is critical for modeling spatial constraints in microfluidic channels.