Gap Closure / Wound Healing or "Scratch" Assay Insert
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Description

This is an insert that can be 3D printed for gap closure assays if you want more consistent gaps than those used by the pipette "scratch" method. These have a little bit of clearance, designed for 14 mm wells. This could be used to create moulds to create silicone/metal or other material inserts suitable to be autoclaved if you want a reusable one that can be easily cleaned. Alternatively, this can be directly used - I recommend sanding the bottom so you can be sure it will lie flush with the bottom of the well-plate.

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Source creator
dagny416
Source license
CC-BY
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Gap Closure / Wound Healing or "Scratch" Assay Insert

From NIH 3D
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Category

learning

License

CC-BY

Published

8/29/2026

Availability

free on NIH 3D