Description

Surface model of the T7 replisome complex as described in Gao et al., Science 10.1126/science.aav7003 (https://science.sciencemag.org/content/sci/early/2019/01/23/science.aav7003.full.pdf) (2019). All components were printed separately and glued. Proteins subunits were printed in PLA, while nucleic (DNA, RNA, and dTTP) in white TPU. The helicases complex from PDB ID 6N7V (https://www.rcsb.org/structure/6N7V) is printed in gray, leading strand polymerase from PDB ID 6N7w (https://www.rcsb.org/structure/6N7W) is printed in green and cooper, lagging strand pol from PDB ID 6N9V (https://www.rcsb.org/structure/6N9V)is printed in red, blue, and yellow. All nucleic acids and relevant ligands were printed in white. RNA was painted black and dTTP was painted blue. An artificial DNA double-helix was generated in Chimera, the two strands were printed separately and used to connect and assemble the subunits (including the lagging strand loop). The same artificial DNA double-helix was printed 2x more times and used to enlarge the template DNA, leading and lagging strands. All printed files are provided as stl. A .py session file usable in Chimera is also provided to help the assembly. The model was created to help me teaching Biochemistry and Molecular Biology at the undergrad level.